Dr. Jitendra Singh Rathore is an Assistant Professor at School of Biotechnology, Gautam Buddha University, Uttar Pradesh, India. He is graduated from University of Delhi, followed by Masters in Biotechnology (M.Sc Biotechnology) from Indian Institute of Technology (IIT) Roorkee. Later, he completed Ph.D in Biotechnology from International Centre for Genetic Engineering and Biotechnology (ICGEB, funded by United Nation) and School of Biotechnology, Jawaharlal Nehru University, (JNU) New Delhi, India.
He joined School of Biotechnology, Gautam Buddha University on 1st Sep 2009. He is recipient of prestigious Fulbright Postdoctoral fellowship from United States-India
2004-2010: Ph.D. in Biotechnology, from School of Biotechnology, Jawaharlal Nehru University (JNU) New Delhi, and International Centre for Genetic Engineering and Biotechnology (ICGEB) New Delhi, India.
2000-2002: M.Sc in Biotechnology, from Indian Institute of Technology (IIT), Roorkee, Uttarakhand, India. (Ist Class)
1999-2000: Post Graduate Diploma in Molecular Biochemical Technology, from Sri Venkateshwara College, University of Delhi, India. (Ist Class)
1996-1999: B.Sc in Life Sciences, from the University of Delhi, India. (Ist Class)
ACADEMICS WORK EXPERIENCE AND INTERNSHIPS
Sep 2014-Till date: Assistant Professor, School of Biotechnology, Gautam Buddha University, (GBU) Greater Noida, U.P, India.
Sep 2013-Aug 2014: Assistant Professor of Microbiology on Visiting Track
Perelman School of Medicine, University of Pennsylvania (UPENN), Philadelphia, USA.
Sep 2013-Aug 2014: Fulbright-Nehru Postdoctoral Fellow, Perelman School of Medicine, University of Pennsylvania (UPENN),Philadelphia, USA.
July 2010- Aug 2013: Assistant Professor, School of Biotechnology, Gautam Buddha University, (GBU) Greater Noida, U.P, India.
Sep 2009-June, 2010: Faculty Associate, School of Biotechnology, Gautam Buddha University, (GBU) Greater Noida, U.P, India.
Nov 2002-July, 2004: Junior Research Fellow, School of Biotechnology, Jawaharlal Nehru University (JNU) New Delhi, India.
May 2001-July 2001: Summer trainee, Department of Biotechnology, All India India Institute of Medical Sciences (AIIMS) New Delhi, India
TEACHING EXPERIENCE (12+: Subjects taught at M.Tech/M.Sc Biotechnology level)
1.Vaccine Design and Development.
4. Genetic Engineering and its Application
5. Introductory Bioinformatics.
6. Industrial Biotechnology.
AWARDS AND ACHIEVEMENTS:
1. "Outstanding Faculty of Science" (Specialization-Biotechnology) from Venus International Foundation in 2019
2. Fulbright-Nehru Postdoctoral Research Fellowship 2013-14 from United States- India Education Foundation (USIEF), USA.
3. Young Scientist Project Awarded under Fast Track Scheme from Science and Engineering Research Board (SERB)-Department of Science and Technology (DST), Government of India, 2011.
4. Selected from Gautam Buddha University as a Teacher to attend 3rd Science conclave (Interaction with Nobel laureates and eminent scientist (An MHRD and DST initiative) 8th Dec-14th Dec 2010 at IIITAllahabad.
Predoctoral travel award from American Society of Cell Biology (ASCB) USA 2007.
Foreign Travel Grant from Council of Scientific & Industrial Research (CSIR) for ASCB conference, 2007.
Foreign Travel Grant from Jawaharlal Nehru University (JNU) corpus fund. 2007.
Senior Research Fellowship (SRF) offered by Council of Scientific & Industrial Research, (CSIR) Govt. of India (Oct 2005-Oct, 2008).
Junior Research Fellowship (JRF) offered by Council of Scientific & Industrial Research (CSIR)Govt. of India (Oct 2003-Oct, 2005).
Qualified National Eligibility Test (NET) for Lectureship conducted by Council of Scientific & Industrial Research (CSIR) Govt. of India, 2002
Qualified National Level GATE examination in 2002.
Studentship for Master offered by Department of Biotechnology, (DBT) Govt. of India (2000-2002)
EDITOR/REVIEWER IN SCIENTIFIC JOURNALS:
Grant Reviewer National Science Centre, Poland.
Reviewer SERB-DST Extramural Individual Centric Research Projects, New Dehli, India.
Evaluator for the proposal under AQIS 2017-18, AICTE, New Delhi.
Ad-hoc reviewer for "BioEssays" Journal WILEY publication
Ad-hoc reviewer for "Clinical Immunology" Journal ELSEVIER publication.
Ad-hoc reviewer for "Innate Immunity" Journal SAGE publication
Editor “Immunology and Infectious Disease” Journal from Horizon Research
publication corporation, USA.
Editor "Mathews Journal of Immunology & Allergy". Mathews International
Editor "Journal of Drug Metabolism & Toxicology": Open access, USA
Editor "EC Pulmonology and Respiratory Medicine" Journal from E-Cronicon publication London, UK.
Editor “Journal of Life Sciences Research” International Journal from Asian online Journal.
Editor “Research and Review: A Journal for Bioinformatics” Journal from STM Journal.
Editor "International Journal of Research in Science & Technology" IRA Publication. 14.Editor "International Journal of Inventions in Engineering & Science Technology" IRA Publication.
Editor "International Journal of Universal Science & Engineering" IRA Publication. 16.Editor "Multidisciplinary International Journal" IRA Publication
M.TECH/M.Sc DISSERTATION/Ph.D. SUPERVISION:
1. M.Tech Dissertation supervised: Thirty two completed (32) and three ongoing (03)
2. Ph.D.: Three (03) ongoing, Two (02) Completed
1. Bacterial Persistence & Host-Pathogen Interaction (Pulmonary Infection) 1. Bacterial Persistence:
Toxin-antitoxin (TA) systems are small genetic elements found on plasmids or chromosomes of countless bacteria, archaea, and possibly also unicellular fungi. Under normal growth conditions, the activity of the toxin protein or its translation is counteracted by an antitoxin protein or non-coding RNA. Five types of TA systems have been proposed that differ markedly in their genetic architectures and modes of activity control. Subtle regulatory properties, frequently responsive to environmental cues, impact the behavior of TA systems. Typically, stress conditions result in the degradation or depletion of the antitoxin. Unleashed toxin proteins impede or alter cellular processes including translation, DNA replication, or ATP or cell wall synthesis. TA toxin activity can then result in cell death or the formation of drug-tolerant persister cells. The versatile properties of TA systems have also been exploited in biotechnology and may aid in combating infectious diseases.
2. Bacterial-Host Interaction (Pulmonary Infection Th17 response)
To perform its primary function of gas exchange, the mucosal surface of the lungs is continuously exposed to the environment. Given the considerable infection risk to the host, the complex mechanism exists to prevent the development of bacterial infection. The immune system in the lungs consists of both innate and adaptive components, and it is clear these two systems are highly interdependent for optimal host defense. With the recent discovery of a distinct subset of Th cells called Th17 cells, in addition to the previously well-characterized Th1- and Th2-cell subsets came many breakthroughs in the realm of innate and adaptive immunity. Th17 cells have been shown to differentiate from nai?ve CD4+ cells in the presence of IL-6 and TGF-? in mice, or IL-6 and IL-1 in humans, when stimulated with appropriate antigen via activation of the transcription factor STAT3. As CD4+ cells commit to the effector Th17 phenotype, the hallmark cytokines IL-17A, IL-17F, IL-21, and IL-22 are expressed
via STAT-3-dependent activation of the critical transcription factor retinoid-related orphan receptor ?t. Th17 cells have been found to play a vital role in host defense against numerous pathogens. However, while Th17 cells are paramount in the adaptive phases of host defense, several other cell types can generate these cytokines in the earlier phases of immune response and bridge the gap between innate and adaptive immunity in the lung. These cell types include ?? T cells, natural killer (NK) cells, NKT cells, and certain innate lymphoid cells.
New vaccine approaches are needed against Streptococcus pneumoniae, Pseudomonas aeruginosa, Bacillus anthracis, and Mycobacterium tuberculosis, which are the keys pathogenic bacteria responsible for serious pulmonary infections in developing countries like India. Although Th17 cells can protect against gram-negative pathogens at mucosal surfaces, including the lung, the bacterial proteins recognized by Th17 cells are largely unknown and could be potential new vaccine candidates. I am interested in the identification of Th17-stimulating antigens from Streptococcus pneumoniae, Pseudomonas aeruginosa, Bacillus anthracis, and Mycobacterium tuberculosis, using ORFeomics approach, which can be used as serotype- independent novel vaccine candidates in the future.
Technical expertise (keyword): Genetic Engineering, Microbiology, Immunology, Bioinformatics, and Proteomics.
RESEARCH GRANTS APPROVED:
1. Transcriptional and Functional Characterization of Xenorhabdus nematophila toxin-antitoxin (TA) modules. Science and Engineering Research Board (SERB)- Department of Science and Technology (DST), Govt of India. Duration Three years (2012-2015) Amount 25 lakh.
2. Transcriptional, functional and persister cell formation studies of novel identified yefM-yoeB, ccdAB and hipAB toxin-antitoxin modules from Xenorhabdus nematophila. Council of Scientific and Industrial Research (CSIR) New Delhi, India. Three years (2016-2022) Amount 30 lakh
3. To decipher the role of genome encoded type II Toxin-Antitoxin (TA) module(s) from Pseudomonas aeruginosa in antibiotic resistance and persister cell formation. Indian Council of Medical Research (ICMR), New Delhi, India Three years (2022-2025)
1. Yadav M, Rathore JS (2021) Functional and transcriptional analysis of chromosomal encoded hipBAXn2 type II toxin-antitoxin (TA) module from Xenorhabdus nematophila. Microb Pathog 2021 Nov 25;162:105309.doi: 10.1016/j.micpath.2021.105309.
2. Garima Singh Mohit Yadav, Chaitali Ghosh and Jitendra Singh Rathore (2021) Bacterial toxin-antitoxin modules: classification, functions, and association with persistence. Current Research in Microbial Sciences 2021 Jul 7;2:100047. doi: 10.1016/j.crmicr.2021.100047. e Collection 2021 Dec.
3. Rathore JS and Ghosh C (2020) Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), a newly emerged pathogen: an overview. Pathog Dis. 2020
4. Nim JS=?, Yadav M=?, Gautam LK, Ghosh C, Sahi S and Rathore JS (2020)
Novel toxin-antitoxin system Xn-mazEF from Xenorhabdus nematophila: identification, characterization and functional exploration. Current Computer Aided Drug Design 2020 Jun 25.doi: 10.2174/1573409916666200625135850
5. Yadav M, Rathore JS (2020) The hipBA(Xn) operon from Xenorhabdus nematophila functions as a bonafidetoxin-antitoxin module. Appl Microbiol Biotechnol. 2020 Feb 11. doi: 10.1007/s00253-020-10441-1.
6. Yadav M and Rathore JS (2018) TAome analysis of type-II toxin-antitoxin system from Xenorhabdus nematophila. Computational Biol and Chem 2018 Oct; 76:293-301
7. Gautam LK, Yadav M, and Rathore JS. (2017) Functional annotation of novel toxin-antitoxin system Xn-RelT of Xenorhabdus nematophila; a combined in silico and in vitro approach. J Mol Model 23 (6): 189
8. Pandey DK, Kumar A, Rathore JS, Singh N, Chaudhary B (2017) Recombinant overexpression of dihydroneopterin aldolase catalyst potentially regulates folate-biofortification. J Basic Microbiol. Apr 18. 57(6):517-524
9. Rathore JS and Yan Wang (2016) Protective role of Th17 cells in pulmonary infection. Review. Vaccine 34(13): 1504-1514
10. GautamLK, YennamalliRM, Rathore JS (2016) Implication on the function of novel Xn-relE toxin structure of Xenorhabdus nematophila using Homology modeling. Current Bioinformatics 12(6): 535 - 542
11. Rathore JS and GautamLK. (2014) Expression, purification and functional analysis of novel relE operon from X. nematophila. The Scientific World Journal Volume 2014 (2014) 428159
12. Rathore JS. (2013) Expression, purification and functional characterization of atypical xenocin, its immunity protein and their domains from Xenorhabdus nematophila. International Journal of Bacteriology Volume 2013 (2013), Article ID 746862
13. Rathore JS. (2013) Function inferences from a molecular structural model of YoeBXn toxin from Xenorhabdus nematophila. American Journal of Bioinformatics Research. 3 (2): 25-29
14. Rathore JS*, Singh MP, and Gautam P. (2013) Insilico analysis of novel hipAB, ccdBA, and yoeB-yefM Toxin-Antitoxin homologs from the Genome of Xenorhabdus nematophila. American Journal of Bioinformatics Research. 3 (2):11-20 (*Corresponding author)
15. Singh, J. (2013) Role of D535 and H538 in endogenous toxicity of xenocin from Xenorhabdus nematophila. FEMS Microbiology Letters 338: p 147-154.
16. Sameer Giri and Singh, J*(2013) New Face in the Row of Human therapeutics: Bacteriocins. Journal of Microbiology Research, 3 (2) p71-78 (*Corresponding author)
17. Singh J. (2012) Structural and Functional Interferences from a Molecular Structural Model of Xenocin Toxin from Xenorhabdus nematophila. American Journal of Bioinformatics Research. 2 (4):p55-60
18. Singh, J*., Chaudhary, R, K., Gautam, P. (2012) Insilico analysis of novel relB, relE and mazF toxin-antitoxin homolog’s from the genome of Xenorhabdus nematophila. 2(3): p21-32. American Journal of Bioinformatics Research (*Corresponding author)
19. Singh, J*. and Ghosh C. (2012) Ribosomal Encoded Bacteriocins: Their functional insight and applications. Journal of Microbiology Research 2(2): p19-25 (*Corresponding author)
20. Singh, J. and Banerjee, N. (2008) Transcriptional analysis and functional characterization of a gene pair encoding iron-regulated xenocin and immunity proteins of Xenorhabdus nematophila. Journal of Bacteriology, 190: p3377-85
21. Banerjee, J.,* Singh, J*, Mohan Chandra Joshi, ShubhenduGhosh, and Nirupama Banerjee. (2006) The Cytotoxic Fimbrial Structural Subunit of Xenorhabdus nematophila Is a Pore-Forming Toxin. Journal of Bacteriology. 188(22): 7957-7962. (*equal contribution).
22. Singh, J., Joshi, M.C., and Bhatnagar, R. (2004). Cloning and expression of glutamine synthetase gene from mycobacterium in E. coli. Biochemical and Biophysical Research Communication. 317: 634-638
1. Chaitali Ghosh, Harish Kumari and Jitendra Singh Rathore, "Prevalence and risk of listeriosis by ready to eat products", International Journal of Scientific Research Letters Vol 1, Issue 1, Dec 2017
2. Sharma P, Ghosh C, Rathore JS. Anthocyanin: Structure, Synthesis and its Beneficial Role in Health. Research & Reviews: Journal of Food Science and Technology. 2016; 5(1): 45–55p.
1. Jitendra Singh Rathore and Chaitali Ghosh "Pathogen-Associated Molecular Patterns and Their Perception in Plants", chapter published in the book titled "Molecular Aspects of Plant-Pathogen Interaction", published by Springer Nature, ISBN 978-981-10-7370-0 ISBN 978-981-10-7371-7 (eBook) 2018.
2. Chaitali Ghosh and Jitendra Singh Rathore "Molecular Biology of Thermophilic and Psychrophilic Archaea" in Microbiomes of Extreme Environments Biodiversity and Biotechnological Applications published by CRC press Taylor and Francis ISBN 9780367342746 November 14, 2020
SEQUENCES SUBMITTED IN NCBI DATABASE:
1. Singh, J. and Banerjee, N. AAZ06276.( 2005). Nucleotide sequence submitted in the NCBI database. Direct submission. Putative colicin like protein [Xenorhabdus nematophila] gi|70671870|gb|AAZ06276.1|
2. Singh, J., and Banerjee, N. AAZ06277. (2005). Nucleotide sequence submitted in the NCBI database. Direct submission. Putative hemagglutinin/hemolysin [Xenorhabdus nematophila gi|120433596|gb|AAZ06 277.1|
3. Singh, J., and Banerjee, N. AAZ06278. (2005). Nucleotide sequence submitted in the NCBI database. Direct submission. Putative cytoplasmic protein [Xenorhabdus nematophila] gi|70671872|gb|AAZ06278.1|
4. Singh, J., Shau, A. K., and Banerjee, N. ABC24675. (2005) Nucleotide sequence submitted in the NCBI database. Direct submission.Glycerophosphoryldiesterphosphodiesteras precursor [Xenorhabdus nematophila]